Abstract—Sixteen Bacillus isolates were obtained from leaves of Miang plant (Camellia sinensis (L.) Kuntze var. assamica (J.W. Mast.) Kitam.) collected from Miang gardens in Chiang Mai and Phrae provinces of Thailand. All bacterial isolates were identified within 3 species including B. licheniformis, B. siamensis and B. tequilensis based on 16S rRNA gene sequence. The culture broth of B. siamensis ML122-2, ML123-1 and ML124-1 could inhibit growth of Staphylococcus aureus ATCC 25923 and methicillin resistant S. aureus DMST 20625 while B. licheniformis ML071-1, ML073-1, ML075-1 and ML076-2 could inhibit growth of B. cereus TISTR 687 and S. aureus ATCC 25923 with the inhibitory value ranging between 242.4 - 363.6 and 265.2 - 340.9 AU/ml, respectively. Moreover, B. siamensis ML122-2 could tolerate tannin, 1% (w/v). Accordingly, B. siamensis ML122-2, ML123-1 and ML124-1 and B. licheniformis ML071-1, ML073-1, ML075-1 and ML076-2 may involve in biological control of Miang fermentation process.

Index Terms—Biocontrol, fermentation, Miang, peptide, tea.

Patthanasak Rungsirivanich is with Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand. He is also with Graduate School, Chiang Mai University, Chiang Mai, 50200, Thailand.
Narumol Thongwai is with Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand. Narumol Thongwai is also with Center of Excellence in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Chiang Mai 50200, Thailand (email: nthongw@hotmail.com; narumol.t@cmu.ac.th).

Cite: Patthanasak Rungsirivanich, Narumol Thongwai, "Antibacterial Activity and Tannin Tolerance of Bacillus spp. Isolated from Leaves of Miang (Camellia sinensis (L.) Kuntze var. assamica (J.W. Mast.) Kitam.)," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 10, no. 1, pp. 26-33, 2020.

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