Abstract—BCSO is effective as an anti-aging agent in cultures of endothelial and macrophage exposed 7.12, dimethyl-benzantracene (DMBA). Reactive radicals damage endothelial tissue, cause inflammatory reactions and have a role in carcinogenesis. Excessive eNOS expression linked to chronic inflammation and carcinogenesis of lung cancer. The research objective was to determine the effect of BCSO on the expression of eNOS and NO levels of macrophage SD rats exposed DMBA.
A study used 36 rats SD that divided into 6 groups, given BCSO (0,01, 0,05 and 0.1 ml / day), positive, negative and normal control. Variables were serum levels of NO and eNOS expression in endothelial tissue.
The results showed that eNOS expression in DMBA group is higher than the normal group (P <0.05). BCSO will decrease eNOS expression and NO levels. ENOS expression and NO levels in group BCSO 0,01 and 0,05 ml / kg body weight / day lower than the DMBA group (p <0.05), and similar to the normal group (P> 0.05). Conclusion: a dose BCSO can degrade endothelial eNOS expression and NO levels of macrophage culture supernatant SD rat DMBA induced.

Index Terms—Antiaging, black cumin seed oil, degenerative diseases, the prevention of cardiovascular disease.

Titiek Hidayati is with Departement of Epidemiology, Community and Family Medicine, Medical and Health Science Faculty, Universitas Muhammadiyah Yogyakarta, Ring Road Barat, Tamantirto, Bantul, Yogyakarta, Indonesia (email: hidayatifkumy@yahoo.co.id).
Akrom is with Departement of Farmacology and Clinical Pharmacy, Prof Supomo Street, Janturan, Umbul harjo, Yogyakarta, Indonesia.
Indrayanti is with Departement of Patology Anatomi, Medical and Health Science Faculty, Universitas Muhammadiyah Yogyakarta, Ring Road Barat, Tamantirto, Bantul, Yogyakarta, Indonesia.
Sagiran is with Departement of Surgery, Medical and Health Science Faculty, Universitas Muhammadiyah Yogyakarta, Ring Road Barat, Tamantirto, Bantul, Yogyakarta, Indonesia.

Cite: Titiek Hidayati, Akrom, Indrayanti, Sagiran, "Evaluation of the Black Cumin Seed Oil Role (BCSO) on a Decline in eNOS Expression and Plasma NO Levels: Initial Studies Kemopreventive BCSO for Lung Cancer," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 7, no. 3, pp. 162-168, 2017.