Abstract—In the present study an attempt was made to examine the potential of different bacterial strains for decolorization of Acid Orange 10, (azo dye) in batch reactors. The effect of media condition, pH, temperature and initial concentration of dye was studied with an aim to determine the optimal conditions required for maximum decolorization and degradation. The bacterial strains used in the study were Pseudomonas putida, Bacillus cereus, Pseudomonas fluorescens, Bacillus subtilis, Alcaligens sp. and Staphylococcus aureus. Out of these Pseudomonas putida emerged out to be most potent decolorizer, being selected for further studies. The selected bacterium shows higher decolorization in static condition as compared to shaking condition. The optimum pH for decolorization of Acid Orange 10 by Pseudomonas putida was 7.0. It shows good decolorization efficiency even in alkaline region. The optimum temperature was 37⁰C. The strain could decolorize Acid Orange 10 (250 mg/l) by 90% within 24 h under the optimum conditions of static condition, pH 7.0, temperature of 37 0C and initial dye concentration of 250 mg/l. The result shows that the selected culture has good potential in removal of Azo dyes from waste water under static conditions.

Index Terms—Biodecolorization, Pseudomonas putida, Acid Orange 10, static condition, Azo dye

The authors are with School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, Varanasi-221005, India(email address: sksribhu@gmail.com).

Cite: A.Tripathi, S.K. Srivastava, "Ecofriendly Treatment of Azo Dyes: Biodecolorization using Bacterial Strains," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 1, no. 1, pp. 37-40, 2011.