Abstract—An experiment was conducted to analyze thei-type lysozyme cDNA cloned from oyster (Crassostrea hongkongensis) and study the effect of temperature on the gene expression in an indoor, controlled constant-temperature environment. The cDNA was 634 bp with a 420-bp open reading frame, encoding 139 amino acids. A new conserved amino acid sequence, HNGGPRGC, was found in the protein sequence of the i-type lysozyme from C. hongkongensis. Real-time quantitative PCR (RTQ-PCR) was used to assess the expression of the i-type lysozyme gene in the digestive gland, smooth muscle, gills, mantle and labial palps of C. hongkongensis. The expression was the highest in the digestive gland and the lowest in the muscle. A thermal stress experiment was performed to study the effect of temperature on the expression of the i-type lysozyme gene. The expression was stable when water temperature ranged from 200C to 250C. The level of expression tended to decrease at 60C or 130C. A conclusion was drawed that the variations in temperature affect i-type lysozyme gene and immune function of C. hongkongensis.

Index Terms—I-type lysozyme, clone, expression, temperature, oyster.

Youqing Xu, Weijun Wu, and Zhaokun Ding are with Institute for Fishery Sciences, Guangxi University, Nanning 530004, Guangxi, China (email: zhaokund@hotmail.com).
Yongmei Li and Weiming Jiang are with Guangxi Institute of Fishery Sciences, 530021 Nanning, Guangxi, China.

Cite: Youqing Xu, Weijun Wu, Zhaokun Ding, Yongmei Li, Weiming Jiang, "Effect of Temperature on the Expression of I-Type Lysozyme cDNA from Oyster (Crassostrea Hongkongensis)," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 6, no. 4, pp. 130-138, 2016.