Abstract—Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Poor understanding of the pathogenesis mechanism of HCC makes it difficult to be diagnosed and treated. The role of microRNAs (miRNAs) associated with progress and metastasis of HCC is emerging recently. For the purpose of systematically understanding the functional regulatory modules of miRNAs in HCC, this study constructed the miRNA-miRNA functional synergistic network (MFSN) based on KEGG pathway functional enrichment of co-targets collected from differentially expressed miRNAs related to HCC. To further understand the complex cooperative relations of miRNAs, two different synergistic subnetworks were generated to exhibit the effect of synergistic suppression of up-regulated miRNAs and synergistic enhancement of down-regulated miRNAs. Moreover, subnetworks of Focal adhesion, MAPK signaling pathway, Wnt signaling pathway, and TGF-beta signaling pathway were also extracted from MFSN to demonstrate synergistic influence profile of miRNAs in a specific functional pathway.