Abstract—In ES cells, many promoters co-modified by the activating H3K4me3 mark and the repressive H3K27me3 mark. These bivalent domains are thought to play a pivotal role related to pluripotency by maintaining lineage commitment programs in a poised state. Enhancers are long-range regulatory elements that orcduring development and differentiation, which are marked by the presence of H3K4me1. However, how enhancers functional interact with bivalent promoters has remained unknown. In this paper, we found bivalent promoters are significantly flanked by poised enhancers which are distinguished by the enrichment of H3K4me1 and the absence of the H3K27ac in hESC. After differentiation of ESC to NPC, a neuron-specific subset of bivalent promoters acquires a chromatin signature with active modification marks.