Abstract—Atherosclerosis as one of the causes of cardiovascular disease will induce endothelial dysfunction and platelet aggregation. Mangostin peel extract (MPE) contains xanthones which have antioxidant activity, anti-cholesterol, anti aggregation, and anti-inflammatory in preventing and inhibiting atherosclerosis. In this research, MPE was evaluated the xanthones quantitative based on standard xanthone compounds using High Liquid Performance Chromatography (HPLC) method and tested anti aggregation platelet activity and ABTS+ 2,2-Azinobis-(3 ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS)-reducing activity. The anti-aggregation platelet using agonist namely adenosine diphosphate inducer (ADP), collagen (COLL), and epinephrine (EPN). Quantification of MPE using four xanthones compound as a marker, showed that MPE contained α-mangostin 105 ppm, γ-mangostin 7.20 ppm, 9.92 ppm Gar-C, and Gar-D 3.50 ppm. MPE and xathones had high ABTS-reducing activity and the most active was , α-mangostin with IC50 2.348 µg/ml. α-mangostin and γ-mangostin had anti-aggregation activity on EPN inducer were comparable with aspirin. MPE and xathones had not anti-aggregation activity on COLL and ADP inducer. MPE contain xathones including α-mangostin, γ-mangostin, Garcinone-C and Garcinone-D. MPE and xanthones have high ABTS-reducing activity. MPE, α-mangostin, γ-mangostin, Garcinone-D decrease EPN-induced aggregation platelet. α-mangostin, γ-mangostin were the most active antiaggregation and antioxidant activities.

Index Terms—Mangostin, Xanthone, Aggregation platelet, Atherosclerosis, Cardiovascular Disease.

Medical Research Center, Faculty of Medicine, Maranatha Christian University, Jl. Prof. Drg. Surya Sumantri 65, Bandung 40164, West Java, Indonesia (email: wahyu_w60@yahoo.com).
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Jl. Babakan Jeruk 2 no 9, Bandung, 40163, West Java, Indonesia.

Cite: Wahyu Widowati, Lusiana Darsono, Jo Suherman, Yelliantty Yelliantty, Maesaroh Maesaroh, "High Performance Liquid Chromatography (HPLC) Analysis, Antioxidant, Antiaggregation of Mangosteen Peel Extract (Garcinia mangostana L.)," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 4, no. 6, pp. 458-466, 2014.