Abstract—Gamma-Oryzanol is one type of antioxidant and has good effect for health. Gamma-Oryzanol can find in rice bran which is the waste from rice milling process. Upland rice is one type of rice which can be grown in the upland area especially in the southern part of Thailand. The upland rice with species ofDawk Pa-yawmhas been used in this research. There are many ways to extract gamma-oryzanol from rice bran. Maceration is conventional and useable extraction method in gamma-oryzanol extraction. Solvent is one crucial parameter in extraction. Many types of organic solvent such as hexane ethyl acetate acetone isopropanol and ethanol are investigated here. The extraction condition such as effect of soaking time, rice and solvent ratio also have examined. The highest amount of rice bran oil obtains from acetone as solvent with the ration of rice bran and solvent is 1:4. Moreover the extracted product is also quality checked by HPLC method. The chromatogram shows clearly the peak of gamma-oryzanol.

Index Terms—Dawk Pa-yawn upland rice, Gamma-oryzanol, maceration, rice bran.

Duangkamol Ruen-Ngam is with the Department of Biology, Faculty of Science, King Mongkut’sInstitute Technology Ladkrabang (KMITL), Bangkok, 10520, Thailand (e-mail: krduangk@kmitl.ac.th, modeliebe@gmail.com).

Cite: Duangkamol Ruen-Ngam, Chitti Thawai, Raumjit Nokkoul, and Sujitra Sukonthamut, "Gamma-Oryzanol Extraction from Upland Rice Bran," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 4, no. 4, pp. 252-255, 2014.