Abstract—Fire blight disease caused by the bacterium Erwinia amylovora is among the most serious threat to rosaceous, was introduced for the first time in Morocco in 2006. Since, the disease propagated then in wide causing immense damages. The objective of this work is the characterization of E.amylovora the population collected between 2006 and 2011. Characterization performed on a collection of 402 strains of E.amylovora from different regions of pome fruit. The obtained results demonstrated a big diversity of the Moroccan collection as well on the phenotypical and biochemical plan, the capacity of certain isolates to degrade the galactose, in particular of the hydrolyseesculin and appearance of colonies on MM2Cu and CCT media. Such as on the molecular plan in particular the ability of multiplex PCR analysis with PEANT1/2, pEA71F/R, EaIsc F/R to identify the E.amylovora strains and the detection of absence of plasmid pEA29 in a few strains.

Index Terms—Erwinia amylovora, rosaceous, biochemical and molecular characterization, Morocco.

Achbani El Hassan is with the Plant Protection URPP- INRA-Meknes Morocco (e-mail: achbani105@gmail.com, dacus5@hotmail.com).
Afaf Ameur, My MustaphaEnnaji, and Naima Rhallabi are with the Laboratory of Virology, Hygiene, Microbiology and Quality/Eco-Toxicology and Biodiversity, Faculty of Sciences and Techniques, Hassan II Mohammedia University–Mohammedia, Casablanca, Morocco (e-mail: afaf.ameur@gmail.com, m.ennaji@yahoo.fr, rallabina@yahoo.fr).
Sophie Cesbron and Charles Manceau are with the Emersysequipe, Research Institute of Horticulture and Seed (IRHS), INRA Beaucouze, Angers, France (e-mail: sophie.cesbron@angers.inra.fr, charles.manceau@anses.fr).

 

Cite: Afaf Ameur, My Mustapha Ennaji, Sophie Cesbron, Charles Manceau, Naima Rhallabi, and El Hassan Achbani, "Characterization of Moroccan Population of Erwinia Amylovora, the Causal Agent of Fire Blight on Rosacous," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 4, no. 3, pp. 200-203, 2014.