Abstract—The Fatty acid-binding proteins (FABP) is a 14-16 kDa conserved multifunctional protein that plays key roles in binding fatty acid ligands, metabolism of fatty acids and metabolic homeostasis. In this study, the complete cDNA of FABP was isolated and characterized from Onychostoma macrolepis to study the functions of FABP in fish metabolism, we report the FABP (GenBank number:JN254629), it contains 405 bp open reading frame(ORF) encoding predicted 134 amino acids. Phylogenetic analysis was showed that Oma-FABP shared 94% sequenced identity with Cyprinus carpio and Rutilus rutilus, suggest that FABP have conserved amino acid sequence and domains in fishes. Expression diversifications of Oma-FABP are analyzed in tissues, feeding stimulation, and starvation challenge using a qRT-PCR approach. In Onychostoma macrolepis, FABP are expressed ubiquitously although transcript levels varied between organs, the highest mRNAs levels are detected in muscle. During feeding stimulation, Oma-FABP was induced and downregulated by dietary protein of 52% in liver ( P <0.05), 42% in intestine (P<0.05). In starvation challenge, Oma-FABP modifies its expression, i ncreasing its transcripts at 2 days, 6 days and 9 days after treatment(P<0.05), the expression of Oma-FABP was highly expressed from 3d to 6d. Overall, these results demonstrate the existence of FABP, and differentially regulated by changes in conditions.

Index Terms—Onychostoma macrolepis, FABP, cloning, qRT-PCR, expression.

Xiaofei Yang, Shaogang Xu, Wentong Li, and Ding Yuan are with the Beijing fisheries research institute, Beijing, China (e-mail: xushaogang@bjfishery.com).
Shiquan Ma is with the Science and Technology Commission of Huairou District, Beijing, China.

 

Cite: Xiaofei Yang, Shaogang Xu, Wentong Li, Ding Yuan, and Shiquan Ma, "Characterization of Gene Expression of Fatty Acid Binding Proteins (FABP) in Largescale Shoveljaw Fish (Onychostoma macrolepis)," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 4, no. 2, pp. 125-129, 2014.