Abstract—Beauveria bassiana is an efficient entomopathogenic fungus for biological control. Optimization on medium for the production of B. bassiana BNBCRC spores in biohydrogen effluent (BHE)-based liquid culture was investigated using Taguchi design. The effects of inorganic and organic nitrogen, trace element as well as the dilution rate of BHE: distilled water were studied in BHE-based composition. The highest spore concentration of B. bassiana BNBCRC was 4.46 ×108 spores mL^-1in the diluted BHE-based medium containing 3.60 g L^-1KNO₃, 4.55 g L^-1 yeast extract and 0.50 g L-1 CaCl₂under the optimal dilution rate of 60:40. The determination coefficient (R²) was 0.99, which ensure an adequate credibility of the model.

Index Terms—Beauveriabassiana, biohydrogen effluent, optimization, taguchi design.

The authors are with the Department of Industrial Biotechnology Faculty of Agro-Industry, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand (e-mail: wanipet@hotmail.com, poonsuk918@yahoo.com).

 

Cite: Wanida Petlamul and Poonsuk Prasertsan, "Medium Optimization for Production of Beauveriabassiana BNBCRC Spores from Biohydrogen Effluent of Palm Oil Mill Using Taguchi Design," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 4, no. 2, pp. 106-109, 2014.