Abstract—The objective of this study was to get the best media to produce somatic embryos from zygotic embryos of sugar palm. This research was conducted at Tissue Culture Laboratory, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University from October 2012 to April 2013. This research consisted of three phases which were callus induction, globular embryo induction, and globular embryo maturation. Sterilization using NaOCl 1.575 % and alcohol 96 % was able to provide the most sterile explants for treatment A1 (MS + 3 mg l ^-1  2,4-D + 2 mg l^-1 NAA) of 89 % (16/18). During callus induction phase, embryogenic callus were mostly formed at treatment A5 (MS + 3 mg l  ^-1   2,4- D + 0.2 mg l ^-1  NAA + 100 mg l^-1 biotin) which is 73 .4 % after 8 weeks. All explants were able to form globular embryos on media B3 (call us A1 to media MS + 4 mg l^-1 Kinetin), B6 (callus A1 to media MS + 1 mg l^-1 Kinetin + 0.1 mg l^-1 TIBA), B12 (callus A2 to media MS + 0.1 mg l^-1 Kinetin + 1 mg l^-1 TIBA), and B14 (callus A2 to media MS + 1 mg l^-1 Kinetin + 1 mg l^-1 TIBA) at 2 weeks after treatment (WAT) during embryo globular ind uction phase. During embryo globular maturation phase, media C11 (globular embryo clumps B9 to media MS + 0.1 mg l^-1 GA3) was able to induce 70.8 % mature globular embryo clumps.

Index Terms—Embryogenesis, globular embryo, plant growth regulators, sugar palm.

Micha Gracianna Devi and Agus Purwito are with Department Agronomy and Horticulture, Agriculture Faculty, Bogor Agricultural University, Indonesia (e-mail: michagrcnndv7@gmail.com).
Ali Husni is with Research and Development Center for Biotechnology and Genetic Resources, Indonesia.

 

Cite: Micha Gracianna Devi, Agus Purwito, and Ali Husni, "Globular Embryo Induction of Sugar Palm (Arenga pinnata (Wurmb) Merr.)," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 4, no. 1, pp. 60-66, 2014.