Abstract—A sterile condition of culture medium is one of the main aspects for micropropagation. The alternative technique for medium sterilization to replace autoclaving was carried out. For sterilization of Murashige and Skoog (MS) medium, commercial essential oils, disinfectants or in combinations were tested. Each essential oil or disinfectant or combination was added to a 20-mL medium in a 120-mL container, kept for 2 weeks before evaluating sterile conditions. Treated media were compared to control medium, autoclaved at 121 degree Celsius for 15 min. Sterile conditions of MS medium were found 100% from 10% povidone-iodine (108 uL), 6% sodium hypochlorite (36 uL), 2% iodine + 2.4% potassium iodide (36 uL), while 95% sterile conditions were obtained from 2% iodine + 2.4% potassium iodide in combination with 10% povidone-iodine (ratio 1 : 1 at 36 uL and ratio 1 : 3 at 72 uL), 10% povidone-iodine in combination with lemon oil (ratio 3 : 1, 108 uL) compared to 100% sterile conditions from autoclaved medium. Effects of these treated media on growth of chrysanthemum shoot and node explants were investigated. It was found that growth of explants on medium treated with 10% povidone-iodine or 2% iodine + 2.4% potassium iodide alone or in combination with 10% povidone-iodine (ratio 1 : 1) or 6% sodium hypochlorite was comparable to those on autoclaved medium.

Index Terms—MS medium, chrysanthemum, disinfectants, essential oils, sterilization, plant tissue culture.

W. Deein, C. Thepsithar, A. Thongpukdee, and S. Tippornwong are with the Biology Department, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand (e-mail: deein_w_su@hotmail.com, tchockpis@gmail.com, chockpis@su.ac.th, toh25@ hotmail.com, ong_kid@ hotmail.com).

 

Cite:Wittaya Deein, Chockpisit Thepsithar, Aree Thongpukdee, and Suppaya Tippornwong, "Growth of Chrysanthemum Explants on MS Medium Sterilized by Disinfectants and Essential Oils," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 6, pp. 609-613, 2013.