Abstract—D6, which is also known as CCBP 2, is one of the decoy chemokine receptors. It was recently found to play a role in the progression of breast cancer cells. In this study, the existence of D6 in invasive breast cancer cells, MDA-MB-231 was investigated by One-step RT-PCR with additional Pfu DNA polymerase in the reaction. The amplicons were then sequenced and compared with the reference sequence from GenBank database. Nucleotide sequence analysis showed that the amplicon sequence matches the reference sequence. Thus, it is confirmed that full length D6 sequence had been amplified from MDA-MB-231.

Index Terms—Cloning, D6, DNA sequencing, MDA-MB-231.

Tan Wee Yee, Chew Ai Lan, amd Khoo Boon Yin are with the Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 USM, Penang, Malaysia (e-mail: wyee129@ yahoo.com, boonyin@usm.my, chew@usm.my; ai_lan@hotmail.com).

Cite:Tan Wee Yee, Khoo Boon Yin, and Chew Ai Lan, "The Construction of Recombinant D6 Clone for in Vitro Breast Cancer Study," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 5, pp. 509-512, 2013.