Abstract—Cellulosic wastes have a lot of uses and able to be converted to useful products such as fuel, chemical feedstock or textiles. Cellulose is a potential substrate to the enzyme that can be produced from the activation of cellulose conversion into monomer. Using microorganisms such as bacteria and fungi as a converter, cellulose can be degraded and converted into reducing sugar with the help of various enzymes including Avicelase enzyme. Enzyme assay is essential to measure enzymatic activity and it is vital for the study of enzyme kinetics and enzyme inhibition. Dinitrosalicylic Colorimetric (DNS) method is used to determine the amount of reducing sugars produced from the enzyme activity on the cellulosic material. From three different sources of isolates, one of the most potent isolate is selected from a food source that is CL5 which has been belonging to Bacillus species by using Gram stain technique. After the optimization, the highest enzyme activity for CL5 can be achieved by monitoring enzyme activity value. The best values resulted at pH 7 with 2.450±0.009 IU/ml, lactose carbon source with 1.286±0.074 IU/ml and potassium nitrate (KNO3) nitrogen source with 2.287±0.109 IU/ml.

Index Terms—Avicelase production, waste management, bacterial isolates, characterization.

N. A. Fauzi and E. A. Makky are with the Industrial Biotechnology, Faculty of Industrial Sciences & Technology, University Malaysia Pahang, Gambang, 26300 Kuantan, Pahang, Malaysia (e-mail: noorafifahfauzi@yahoo.com, essam22001@gmail.com).

Cite:N. A. Fauzi and E. A. Makky, "Avicelase Enzyme from Sawdust: Isolation, Production and Optimization," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 5, pp. 501-504, 2013.