Abstract—A rapid and sensitive detection method is proposed to evaluate the presence and vitality degree of Anisakidae larvae in marine fishes by UV diaphanoscopy. 923 fish samples belonging to seven fish species (Merluccius merluccius, Molva elongata, Sardina pilchardus, Scomber scomber, Triglia lucerna, Engraulis encrasicolus, and Trachurus trachurus) were evaluated for the presence of Anisakidae larvae by using direct observation, peptic digestion, and UV transillumination. The results obtained indicate that UV transillumination can be considered a rapid and accurate tool for routine analysis of marine fishes even under field condition.

Index Terms—Anisakidae, larvae, detection, UV, transillumination.

G. V. Celano is with the Veterinary Medicine Department, University of Bari, Italy (email: gaetanovitale.celano@uniba.it).
A. Paparella is with the Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Italy (email: apaparella@unite.it).
A. Fransvea and G. Celano are with Med & Food C.Q.S. Srl, Valenzano (BA), Italy (email: armidafransvea@gmail.com, g.celano1@gmail.com).
C. Balzaretti is with the Department of Veterinary Science for Health, Animal Production, and Food Safety, University of Milan, Italy (email: claudia.balzaretti@unimi.it).

Cite:Gaetano Vitale Celano, Antonello Paparella, Armida Fransvea, Claudia Balzaretti, and Giuseppe Celano, "Rapid Method for Detection of Anisakidae Larvae in Marine Fishes, Based on UV Transillumination," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 4, pp. 392-394, 2013.