Abstract—This study investigates the extraction of the Vernonia cinerea using supercritical carbon dioxide extraction under the following conditions pressure at 300, 400 and 500 bar, temperatures at 313, 323 and 333 K, CO₂ flow rate at 2 and 3 L/min. The best operation of extraction is under 500 bar, 333 K and 3 L/min CO₂ flow rate. The reverse-phase high performance liquid chromatography method was developed for determining major active components of Vernonia cinerea. We dissolve the sample with methanol and analyzed by using a MetaChem C18-A(250 × 4.6m, 5μ) column with a gradient elution composed of ultra-pure water and acetonitrile. The wavelength of UV detector was set at 280 nm and the flow-rate was set at 1 mL/min. Under this condition,

Index Terms—Vernonia cinerea, supercritical carbon dioxide, luteolin, flavonoids.

Guan-Rui Lai is with Ming Chi University of Technology, Department of Chemical Engineering, TW (e-mail: qooforme@yahoo.com.tw).

Cite:Guan-Rui Lai and Chiao-Song Wu, "Analyzing of Major Active Luteolin in Vernonia Cinerea," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 4, pp. 363-367, 2013.