Abstract—A lectin has been isolated from phaseolus vulgaris and purified by ion exchang and gel filtration . The purified lectin was immobilized with glutaraldehyde. The in vitro interaction of free and immobilized lectin with nine pathogenic bacteria ( E. coli , Bacillus , Enterobacterium , Salmonella , Gardenella , Staphylococcus ,Streptococcus , Morganella , Pseudomonas ) were study . The result showing agglutination of six pathogenic bacteria ( Bacillus , Enterobacterim , Salmonella , Staphylococuss , Streptococus , Pseudomonas ) with free lectin. Glutaraldehyde immobilized lectin purified from Phaseolus vulgaris resulted in an insoluble polymer suspension. The interaction of lectin polymer suspension with liquid culture of pathogenic bacteria was quantified by measuring the OD (660 nm) at time zero and after ten minutes from incubation.
The results showed strong agglutination of immobilized lectin with Bacillus, Staphylococcus, Streptococcus, and Pseudomonas more than agglutination with E. coli, Enterobacterium, Morganella and Gardenella.

Index Terms—Immobilization, lectin, lectin-bacteria interaction.

Mohammed A. Jebor is with Babylon University, College of Science, Biology Department (e-mail: Mohammed622005@Yahoo.com).

 

Cite:Mohammed A. Jebor and Yasser H. Jalil, "Interaction of Some Pathogenic Bacteria with Phaseolus Vulgaris L. Seeds Lectin (as Biosensor)," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 3, pp. 239-241, 2013.