Abstract—Cigarette smoke (CS) is released into the atmosphere, and impact lung health in non-smoker but not smoker. CS is inhaled into the lung by respiration and affects alveolar macrophages (AM). AM play an important role of immune system in the lung. In this study, we investigated the effect of CS on DNA damage and immune function in AM. The number of AM was significantly increased in CS exposed mice compared with non CS-exposed mice. Expressions of CD11b, TLR-2 and CD14 on AM were significantly inhibited in CS exposed mice but not CD16. Phagocytic activity of AM was significantly inhibited in CS exposed mice. Both of tail moment and tail length of AM as indicator of DNA damage were significantly increased in CS exposed mice. CS was a risk factor for DNA damage of AM and induced inhibition of immunological functions in AM mediated with DNA damage. These results suggest that changes of intracellular structure, inhibition of phagocytosis and TLR expression and induced-DNA damage of AM by CS may result in easily infection of bacteria or virus and carcinogenesis.

Index Terms—Cigarette smoke, alveolar macrophages, DNA damage, phagocytic activity

Yuriko Hirono and Minoru Takeuchi are with the Faculty of Life Sciences, Kyoto Sangyo University, Kyoto, Japan (e-mail: s4373@cc.kyoto-su.ac.jp, mtakex@cc.kyoto-su.ac.jp).

 

Cite:Yuriko Hirono, Ayaka Kawazoe, Masahiko Nose, Masaaki Sakura, and Minoru Takeuchi, "Cigarette Smoke Induce Alteration of Structure and Function in Alveolar Macrophages," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 2, pp. 125-128, 2013.