Abstract—PP2C is a negative regulator of ABA dependent abiotic stress pathway. Genome wide analysis of Arabidopsis thaliana revealed that PP2C like promoter (AT5G59220) has both biotic and abiotic responsive cis elements in the upstream of the TATA box. In this study, full length and deletion variants of PP2C like promoter were cloned upstream of the egfp reporter gene and bombarded on to the tobacco leaves. The -400 bp deleted construct showed maximal expression of the reporter gene.

Index Terms—ABA, cis elements, EGFP, PP2C

The authors are with the Department of Biological Sciences, Faculty Division III, BITS Pilani, Pilani Campus (e-mail: purvalohan@gmail.com).

 

Cite:Purva Bhalothia, Sandhya Mehrotra, and Rajesh Mehrotra, "Cloning and Characterization of Protein Phosphatase 2C (PP2C) like Promoter from Arabidopsis Thaliana," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 2, pp. 103-105, 2013.