Abstract—The complete genome sequence of V. cholerae has revealed the presence of four CheV’s, namely putative CheV, CheV1, CheV2 and CheV3, along with the three chemotaxis-related signaling systems designated as System I, II and III. To determine the roles of the CheV proteins, whose genes are located outside of the che gene clusters, we examined their cellular localization and then their roles in chemotaxis. Among the four CheV’s, CheV1 plays a major role in chemotaxis as observed with swarm assay and fluorescence microscopy, suggesting its involvement in System II.

Index Terms—Chemotaxis, fluorescence microscopy, fusion proteins, localization, signal transduction, swarm assay.

Geetha Hiremath is with the Research center for Micro-Nano technology, Hosei university, Japan (email: geetha.2225@gmail.com).
So-Ichiro Nishiyama is with Dept. of Frontier Bioscience, Faculty of Bioscience and Applied Chemistry, Hosei University, Japan.
Ikuro Kawagishi is with Dept. of Frontier Bioscience, Graduate School of Engineering, Hosei University.

 

Cite:Geetha Hiremath, So-Ichiro Nishiyama, and Ikuro Kawagishi, "CheV1 Plays an Important Role in Chemotaxis of Vibrio Cholerae," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 2, pp. 71-74, 2013.