Abstract—Flavonoids content of two types of hawthorn leaves (crataegus azarolus) which grows in Iraqi Kurdistan region (season 2011) including Zalan and Qaradax, were determined by RP-HPLC technique. The percentages of major flavonoids contents were found to be Hypersoide and Vitexin-rahmnose(28.12-30.26 %), ( 30.30-18.00%), respectively and these results were compared with ratio of them in the hawthorn fruits of the same plants in which these two majors (Hypersoide and Vitexin-rahmnose) flavonoids became very low but some other flavonoids like Querecetin, Nonacosan-10-01 and Apigenin show high percentage (18.24%, 13.72% and 11.98% respectively) in Hawraman hawthorn and in Qaradax hawthorn some other types are dominated like Nonacosan-10-01, Kaempferol and Querecetin having following ratio (18.10%, 16.59% and 15.51% respectively). Divert compounds of flavonoids composition were analyzed also by HPLC technique in these two types of hawthorn leaves, different ratio of : 1- Neoategolic acid, 2-Kaempferol (I), 3-Querecetin, 4-Nonacosan10-01 (II), 5-Apigenin(III), 6-Luteolin(IV), 7-Rutin(V), 8-Hypersoide(VI) and 9-Vitexin-rahmnose (VII), these compounds have main physiological role and pharmaceutical effect on the fruits of Hawthorn and also same role on the animal and human physiology.

Index Terms—Crataegus Azarolus, flavonoids, HPLC analysis, Hawthorn.

Baram Ahmed Hamahameen is with the Department of Chemistry, School of Science, Faculty of Science&Science Education, University of Sulaimani (e-mail: baramjaff@yahoo.com).
Banaz Jamal is with the Department of Field Corps, Faculty of Agriculture, university of Sulaimani

Cite: Baram Ahmed Hamahameen and Banaz Jamal, "Determination of Flavonoids in the Leaves of Hawthorn (Crataegus Azarolus ) of Iraqi Kurdistan Region by HPLC Analysis," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 1, pp. 67-70, 2013.