Abstract—Study of biodelignification of rice straw was investigated by using a fungus isolated from indigenous the straw. Identification of the fungus was characterized by 18s rRNA sequencing and included in the new species of Penicillium sp. strain apw-tt2. Ligninase production and cell growth was observed in incubation condition of Submerged Fermentation (SmF). Optimizations of the enzyme production condition were carried out by altering pH and temperature of the incubation medium. The results showed that the optimum conditions for biopulping are at 40˚C and pH 5. High ligninase production was observed at Day 4 with activity 571 U/ml with degraded lignin 66.3%. The quality of pulp showed that the new species is very effective in delignifying rice straw for pulping.

Index Terms—Penicillium sp., delignification, 18s rRNA, ligninase, biopulping

Asri Peni Wulandari is with the Universitas Padjadjaran, Indonesia (e-mail: asri.peni@unpad.ac.id).
Tika Triyana and Poniah Andayaningsih are with the Department of Biology, Universitas Padjadjaran.

Cite: Asri Peni Wulandari, Tika Triyana, and Poniah Andayaningsih, "Delignification of Rice Straw with Ligninase from Novel Penicillium sp. strain apw-tt2 for Biopulping," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 3, no. 1, pp. 43-46, 2013.