Abstract—Guibi-tang (GB) is a traditional medicine used for anti-oxidant, anti-osteoporosis, hemostasis and gastroprotection. We investigated the levels of several compounds in GB before and after fermented with Lactobacillus curvatus KFRI 166 (GB166) and Lactobacillus plantarum KFRI 442 (GB442). The characterization of seven compounds were achieved by comparing the HPLC retention times (tR) and UV absorptions of target peaks in the GB with those of standards liquiritin (1), nodakenin (2), nodakenitin (3), decursinol (4), 6-gingerol (5), decursinol angelate (6) and decursin (7). We are special ingredients of GB166 and GB442 were compared. As a result, the five compo-unds with the GB166 was decreased by L. curvatus KFRI 166, and the other nodakenin (2) and decursinol (4) were fermentation selective increased. But, GB442 was all compounds decreased. GB fermented with Lactobacillus affects the content of several compounds will be improves its absorption for develop the bioactivity.

Index Terms—Guibi-tang, fermented, analysis, HPLC

The authors are with the Korea Institute of Oriental Medicine, KM-based herbal drug research, 1672 Yuseongdaero, Yuseong-gu, Daejeon (e-mail: spring172@kiom.re.kr, leekj@kiom.re.kr, jsw9093@kiom.re.kr, hahaha917@kiom.re.kr, jyma@kiom.re.kr; Phone: +821073981994).

Cite: C. Liang, K. J. Lee, S. W. Jeong, J. H. Ha, and J. Y. Ma, "Analysis of Constituents from Guibi-Tang with Lactobacillus," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 2, no. 6, pp. 374-376, 2012.