Abstract—Anthracnose is the most important fungal disease on Persian walnut in Iran. The current study was performed on 16 Ophiognomonialeptostyla isolates collected from northwest region of Iran. ITS amplification was performed by using ITS1 and ITS4 primers and the sequences were analyzed. Phylogenetic trees are made based on ITS sequences which showed that all Iranian isolates are in the same branch in a clade with Ophiognomonialeptostyla from CBS isolated from Juglansregia. The Iranian isolates are divided into two closely related sub-clades with low bootstrap support, the smaller sub-clade comprised of only three isolates were collected from wild walnut trees located in woodland areas.

Index Terms—Gnomonialeptostyla, ITS sequencing, phylogentic tree, walnut leaf blotch.

Soleiman Jamshidi is with the Department of Plant Protection, Islamic Azad University, Miyaneh Branch, Miyaneh, Iran (e-mail: s.jamshdi@m-iau.ac.ir).
Rasoul Zare is with the Department of Botany Iranian Research Institute of Plant Protection, Tehran, Iran (e-mail: simplicillium@yahoo.com).

Cite: Soleiman Jamshidi and Rasoul Zare, "Molecular Phylogeny of Ophiognominialeptostyla Isolates Collected from Iran Based on ITS nrDNA Sequences," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 2, no. 6, pp.  438-440, 2012.