Abstract—Goal of present studies was the optimization of production media of D-amino acid oxidase by Trigonopsis variabilis using one-at-a-time strategy & screening of novel inducer. In different carbon and nitrogen sources, glucose and ammonium sulfate were found the best carbon and nitrogen source with 4:1 C: N ratio. The best new inducers found were 3-(3, 4 -Dihydroxyphenyl) DL-alanine, DL-β- Phenyl alanine, DL-2- Amino-n-butyric acid. Four fold of D-amino acid oxidase yield was observed when 3-(3, 4 -Dihydroxyphenyl) DL-alanine was used as inducer as compared with D-Ala. The optimum concentration of the best inducer 3-(3, 4 -Dihydroxyphenyl) DL-alanine, was 10 mM. High Induction effect was observed when inducer was added in media in 0 hour of fermentation.

Index Terms—D-Amino acid oxidase (DAAO), 3-(3, 4Dihydro -xyphenyl) DL-alanine, Trigonopsis variabilis

The authors are with School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, Varanasi-221005, India (e-mail: debnathmira@yahoo.co.in, neeraj.rs.bce@itbhu.ac.in)

Cite: Neeraj Gupta, Ravi Kumar Gundampati, and Debnath M., "Screening of Novel Inducer for D-amino Acid Oxidase by Trigonopsis variabilis," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 2, no. 3, pp. 200-202, 2012.