Abstract—This study presented the feasibility of themicroparticle manipulation, i.e., trapping and separation approach, utilizing an electrodeless dielectrophoresis microfluidic chip. Experimental observations were facilitated toinvestigate the microparticle electrokinesis behavior by tuningof the operation parameters, i.e., driving frequency and appliedpotential. These results not only revealed the fluid cloud begenerated an electroosmotic flow (EOF), but also categorizedthe optimum trapping/separating schemes for the differentparticle sizes. Besides, the proposed operating mode can be usedto continuously trap/separate particles and simultaneouslyconvey them through the microfluidic device, eliminating theneed for an additional micropump. Certainly, the device can beenvisioned as a single automated device that supports themulti-functions such as transportation, separation, anddetection, facilitating the realization of a Lab-on-a-Chip.

Index Terms—trapping, separation, electrodelessdielectrophoresis, electroosmosis, dielectrophoresis.

J.-L. Lin is with Department of Mechanical and Automation Engineering,I-Shou University, Kaohsiung , Taiwan (tel.: +886-7-6577711 Ext3320;e-mail: ljl@isu.edu.tw.) .
L.-J. Chien and C.-H. Chiou both served as a principal investigator inMicrosystems Technology Center at ITRI South, Industrial TechnologyResearch Institute, Taiwan (e-mail: liangju95@gmail.com,prayjohn@itri.org.tw )
C.-Y. Lee, J.-C. Pan, and Y.-L. Gao all are in Department of Mechanicaland Automation Engineering, I-Shou University, Kaohsiung, Taiwan(e-mail: sken662002@yahoo.com.tw, william@major.com.tw andisu9904005m@isu.edu.tw).

Cite: Liang-Ju Chien, Chen- Yu Lee, Jia-Cheng Pan, Yao-Lin Gao, Chi-Han Chiou, and Jr-Lung Lin, "Potential Application for Micro-Particles Manipuation Utilizing Electrokinesis in an Electrodeless Dielectrophoresis Chip," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 2, no. 3, pp. 174-178, 2012.