Abstract—Plant tissue culture technique combined with elicitors is required to produce plant secondary metabolites. The results showed that both elicitors (methyl jasmonate and salicylic acid) could enhance the production of Stemona alkaloids in the Stemona sp. culture. Among these elicitors, salicylic acid was found to be the most effective elicitor. The plantlet which was elicited with 100 μM salicylic acid for 1 week showed an increase of 1′,2′-didehydrostemo-foline and stemofoline production at 1.69 fold and 1.61 fold higher than the control, respectively.

Index Terms—1′,2′-didehydrostemofoline, stemofoline, methyl jasmonate, salicylic acid.

N. Chaichana and S. Dheeranupattana are with Department of Biology, Chiang Mai University, Chiang Mai 50200, Thailand (e-mail: nat_too@hotmail.com).

Cite: Natthiya Chaichana and Srisulak Dheeranupattana, "Effects of Methyl Jasmonate and Salicylic Acid on Alkaloid Production from in vitro Culture of Stemona sp.," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 2, no. 3, pp. 146-150, 2012.