Abstract—Arthrobotrys musiformis is a biocontrol agents which can be used in the integrated pest management strategies. Although this isolate was found to show high benzimidazole resistance as compared to the other biocontrol agents, considering the dose of administration of anthelmintics, a need for developing an improved strain of this fungus resistant to chemical anthelimintics was observed. In the present study improved strains of Arthrobotrys musiformis were obtained using random mutagenesis technique carried out both by chemical mutagenic agent, Ethyl methane sulfonate (EMS) and physical mutagenic agent, UV radiation. The obtained mutants were screened using their ability to survive in high benzimidazole concentration, isolated, selected based on their biocontrol activity and characterized for their suitability as biocontrol agents. The β-tubulin genes of the selected mutants were finally studied.

Index Terms—Arthrobotrys musiformis, benzimidazole resistance, beta-tubulin genes, biocontrol agents, mutagenesis.

Bushra S. Jarullah is with the Department of Biotechnology, Kadi Sarva Vishwavidyalaya, Gandhinagar, Gujarat, India (phone: +91-9824084524; e-mail: bjarullah@ yahoo.com).
R. B. Subramanian is with BRD School of Biosciences, S P University, Vallabh Vidyanagar, Gujarat, India (e-mail: subramanianrb@gmail.com).
S. Kajal was with the BRD School of Biosciences, S P University, Vallabh vidyanagar, Gujarat, India. He is now with the ARIBAS, S P University, Vallabh vidyanagar, Gujarat, India (e-mail: sushil14_2001@yahoo.com).
J. S. Jarullah is with the King Fahd Medical Research Center, King Abdulaziz University, Jeddah, KSA (e-mail: jumanaj@yahoo.com).

Cite: Bushra S. Jarullah, R. B. Subramanian, S. Kajal, and J. S. Jarullah, "Comparative In-Silico Study of β-Tubulin Proteins from Arthrobotrys musiformis for Resistance to Benzimidazole," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 2, no. 2, pp. 131-135, 2012.