Abstract—Aquatic fungi of the genus saprolegnia often cause serious damage to fresh water fish such as rainbow trout. Malachite green is quite an effective antifungal agent but it is teratogenic, mutagenic and carcinogenic. Other antifungal agents like hydrogen peroxide, formalin, sodium chloride, have some negative points, so an alternative antifungal agent is needed to be effective and safe, not synthetic but natural substance like plant metabolits. Ruta graveolens is an herbal plant which is traditionally used as an energizer and an anti-bleeding to heal injuries. Many of Ruta species contain various natural components that are anti-fungal and phytotoxic. In this experimental research antifungal effect of ethanolic extract of Ruta graveolens root on saprolegnia, in vitro by diffusion tests (disk and well) and dilution tests (MIC and MFC nomination) was studied. It was shown that root ethanolic extract of Ruta graveolens had antifugnal effects and prevented from saprolegnia growth .MIC of ethanolic extracts was determined to be 25×10³ μg/ml.

Index Terms—Saprolegnia, Ruta graveolens, Antifungal effect

Hashemi Karouei S. M. is with Department of Mycology, Tonekabon Branch Islamic Azad University, Iran (e-mail: m.hashemi@toniau.ac.ir).
Sadeghpour Haji M. is M.Sc in microbiology
Gholampour Azizi I. is with Department of Mycology, Babol Branch Islamic Azad University- Babol, Iran.

Cite: Hashemi Karouei S. M., Sadeghpour Haji M., and Gholampour Azizi I., "Isolation of Saprolegnia and the Influence of Root Ethanolic Extract of Ruta graveolens on Saprolegnia. Spp Growth," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 2, no. 1, pp. 64-67, 2012.