Abstract—Phyllanthus amarus plant is used in traditional system of medicine as hepatoprotective drug for which the major lignans phyllanthin and hypophyllanthin are responsible. As so far no significant work has been done on culture aspect of this plant, hence realizing the hepatoprotective potential, the present investigation was undertaken.
A cost effective process was developed for enhancing phyllanthin and hypophyllanthin utilizing immobilization technique. HPTLC was used to compare phyllanthin and hypophyllanthin contents in calcium alginate immobilized cells obtained from fresh grown plants and M. S. medium was supplemented with different precursors and phytohormones, for treatment with kinetin, napthalene acetic acid, chitosan and cinnamic acid solution to make whole process commercially viable. It was revealed that cinnamic acid and chitosan in low concentration enhances phyllanthin and hypophyllanthin yield as compared to control. The study revealed that increase in the content of phyllanthin and hypophyllanthin was precursor concentration dependent and cinnamic acid treatment give maximum yield of hepatoprotective bioactives as compared to other precursor and phytohormones used.

Index Terms—Hepatoprotective, HPTLC, Immobilization, Precursor.

Authors are with Department of Pharmaceutical Sciences, Dr. Hari Singh Gour Central University, Sagar (M.P.) 470003 India (Tel.: 011-7582 -222554; fax: 011-7582-230063; e-mail: jaiwantthakur@gmail.com).

 

Cite: J. S. Thakur and M. D. Kharya, "Enhancing Hepatoprotective Bioactive's from Phyllanthus Amaraus Through Immobilization," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 1, no. 4, pp. 302-306, 2011.