Abstract—In order to diagnose MOTT species M. smegmatis and M. kansasii along with M. tuberculosis, species specific primers were designed using PRIMER-BLAST which amplified particular 16S rRNA genes having variable amplicons sizes. All primer sets were found to be specific for their target 16S regions of mycobacterium species. Easy and fast DNA isolation protocol developed that could be implemented directly for PCR reaction of the samples. By involving standardized PCR conditions with defined primers and genome combinations, we have successfully reported the 16S rRNA gene based PCR detection of several mycobacterium species which has highlighted the MOTT detection capabilities.

Index Terms—MOTT, Mycobacterium species, PCR, 16SrRNA.

The authors are with Biotechnology and Microbiology department of G. H. Raisoni institute of Interdisciplinary Sciences Nagpur (email: acmedilip4u@yahoo.com)

Cite: Niharika M. Amrute, Atul P. Denge, Dilip G. Gore, and Suchitra S. Godbole, "Identification of Several Mycobacterium Species by using Amplified 16S Ribosomal DNA," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 1, no. 3, pp. 219-222, 2011.