Abstract—Achillea wilhelmsii is belong to Asteraceae family. This plant is from medicinal plants in Iran customary medicine.The aim of this study was to appraise of effects of the leaves essential oil and methanol extract of the Achillea wilhelmsii on the growth of the bacteries. In this study after collecting and provision plant, leaves essential oil were obtained by hydrodistillation and leaves methanol extract were obtained using a Soxhlet apparatus. The effects of methanol extract antimicrobial were assessed using Agar Well Diffusion method and also using Dilution Test method. The effects of essential oil antimicrobial were determined by using Agar Dilution Assay. Dates were analyzed using Chi-square and AVONA test in the p <0/001.Leaves methanol extract had more effect against Bacillus cereus and Staphylococcus aureus bacteries and showed weak effect against Escherchia coli and was not abserved any growth inhibition effect against Pseudomonas aeroginosa,also leaves essential oil had inhibition effect against Bacillus cereus, Staphilococcus aureus and Escherchia coli and did not have any inhibition effect against Pseudomonas aeroginosa.Leaves methanol extract and essential oil of Achillea wilhemsii have antibacterial effects,therefore we will be able perform researches with extraction of this plant effective compound for the treatment of infectious diseases.

Index Terms—Achillea wilhelmsii, antibacterial effect, essential oil, ,methanol extract.

L. A. is with the Department of Biology, Falavarjan Branch, Islamic Azad University, Esfahan-Iran ( Corresponding auther, telefax: +98-312-312-0136; e-mail: amjad.leila@ gmail .com or amjad@iaufala.ac.ir).
M. Ms. is with the Department of Microbiology, Falavarjan Branch, Islamic Azad University, Esfahan-Iran (e-mail: mohammadis_m@ yahoo.com or mohamadi_m@iaufala.ac.ir).
M. Mka. Author is with the Young Researchers Club, Falavarjan Branch, Islamic Azad University, Esfahan-Iran (e-mail: min_mohammady_1365@ yahoo.com).

Cite: L. Amjad, M. Mohammadi-Sichani, and M. Mohammadi-Kamalabadi, "Potential Activity of the Achillea wilhelmsii Leaves on Bacteria," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 1, no. 3, pp. 216-218, 2011.