Abstract—Mechanism of viral infection is not apart from role and function of grouper receptor, especially brain defence which mediated by nervous cells, heart and kydney. This research is to identify how a role and expression of CD4 and CD8 cells to defence of VNN infection in C. altivelis. Receptor protein in brain, heart and kidney organ from C.altivelis infected by VNN were isolate, identify and charaterize using SDS-Page. The expression of CD4 and CD8 cells on receptor protein grouper observed by Confocal Laser Scanning Microscope (CLSM) and the response and specifity of receptor protein shown by dot blotting and western blotting respectively. Cross reactivity of specifity of organ showed by imunocytochemistry and immunohistochemistry methods. The result indicate that receptor protein which is in nervous cells on brain organ, heart and kidney organ by molecule weight 32.5 kDa is receptor protein expressed on nervous cells on brain organ, heart and kidney of C. altivelis. It give a response to recognize VNN infection evidenced by antibody yield which have cross reaction through dot blotting, western blotting inspection and expression of receptor showed by fluorescence color in CLSM inspection and immunocytochemistry and immunohistochemistry. Protein of nervous cells on organ of brain, heart and kidney of C. altivelis by 32.5 kDa is a receptor protein which have function to recognize antigen VNN and the roles for expression of CD4 and CD8 cells.

Index Terms—C. altivelis, CD4, CD8, VNN.

Uun Yanuhar is with Faculty of Fisheries and Marine Science, University of Brawijaya, East Java, Indonesia; corresponding author to provide phone: +6281931892262; e-mail:uunyanuhar@ yahoo.com.

Cite: Uun Yanuhar, "The Function of Receptor Protein Humpback Grouper Cromileptes Altivelis in Expression and Proliferation of CD4 and CD8 cells in Defence Immunity of Viral Nervous Necrotic Infection," International Journal of Bioscience, Biochemistry and Bioinformatics vol. 1, no. 2, pp. 119-124, 2011.